Research Groups

 

Publications of Klaas Martinus Pos (Results: 56)

 

Mechanisms of envelope permeability and antibiotic influx and efflux in Gram-negative bacteria.
Nature microbiology
Masi M, Réfregiers M, Pos KM, Pagès JM PubMed
Feb 2017 Volume: 2 Issue: Pages: 17001

Cytochrome c Oxidase Biogenesis and Metallochaperone Interactions: Steps in the Assembly Pathway of a Bacterial Complex.
PloS one
Schimo S, Wittig I, Pos KM, Ludwig B PubMed
2017 Volume: 12 Issue: 1 Pages: e0170037

Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Proceedings of the National Academy of Sciences of the United States of America
Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampé R PubMed
Jan 2017 Volume: 114 Issue: 4 Pages: E438-E447

Transport of lipophilic carboxylates is mediated by transmembrane helix 2 in multidrug transporter AcrB.
Nature communications
Oswald C, Tam HK, Pos KM PubMed
Dec 2016 Volume: 7 Issue: Pages: 13819

Editorial: Bad Bugs in the XXIst Century: Resistance Mediated by Multi-Drug Efflux Pumps in Gram-Negative Bacteria.
Frontiers in microbiology
Vargiu AV, Pos KM, Poole K, Nikaido H PubMed
2016 Volume: 7 Issue: Pages: 833

Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives.
Proceedings of the National Academy of Sciences of the United States of America
Sjuts H, Vargiu AV, Kwasny SM, Nguyen ST, Kim HS, Ding X, Ornik AR, Ruggerone P, Bowlin TL, Nikaido H, Pos KM, Opperman TJ PubMed
Mar 2016 Volume: 113 Issue: 13 Pages: 3509-14

Tripartite assembly of RND multidrug efflux pumps.
Nature communications
Daury L, Orange F, Taveau JC, Verchère A, Monlezun L, Gounou C, Marreddy RK, Picard M, Broutin I, Pos KM, Lambert O PubMed
Feb 2016 Volume: 7 Issue: Pages: 10731

Molecular basis of polyspecificity of the Small Multidrug Resistance Efflux Pump AbeS from Acinetobacter baumannii.
Journal of molecular biology
Lytvynenko I, Brill S, Oswald C, Pos KM PubMed
Feb 2016 Volume: 428 Issue: 3 Pages: 644-57

BGA66 and BGA71 facilitate complement resistance of Borrelia bavariensis by inhibiting assembly of the membrane attack complex.
Molecular microbiology
Hammerschmidt C, Klevenhaus Y, Koenigs A, Hallström T, Fingerle V, Skerka C, Pos KM, Zipfel PF, Wallich R, Kraiczy P PubMed
Jan 2016 Volume: 99 Issue: 2 Pages: 407-24

Structure, mechanism and cooperation of bacterial multidrug transporters.
Current opinion in structural biology
Du D, van Veen HW, Murakami S, Pos KM, Luisi BF PubMed
Aug 2015 Volume: 33 Issue: Pages: 76-91

Correction to Approved Drugs Containing Thiols as Inhibitors of Metallo-β-lactamases: Strategy To Combat Multidrug-Resistant Bacteria.
Journal of medicinal chemistry
Klingler FM, Wichelhaus TA, Frank D, Cuesta-Bernal J, El-Delik J, Müller HF, Sjuts H, Göttig S, Koenigs A, Pos KM, Pogoryelov D, Proschak E PubMed
Aug 2015 Volume: 58 Issue: 15 Pages: 6320

The assembly and disassembly of the AcrAB-TolC three-component multidrug efflux pump.
Biological chemistry
Müller RT, Pos KM PubMed
Sep 2015 Volume: 396 Issue: 9-10 Pages: 1083-9

Approved Drugs Containing Thiols as Inhibitors of Metallo-β-lactamases: Strategy To Combat Multidrug-Resistant Bacteria.
Journal of medicinal chemistry
Klingler FM, Wichelhaus TA, Frank D, Cuesta-Bernal J, El-Delik J, Müller HF, Sjuts H, Göttig S, Koenigs A, Pos KM, Pogoryelov D, Proschak E PubMed
Apr 2015 Volume: 58 Issue: 8 Pages: 3626-30

A novel packing arrangement of AcrB in the lipid bilayer membrane.
FEBS letters
Ly K, Bartho JD, Eicher T, Pos KM, Mitra AK PubMed
Dec 2014 Volume: 588 Issue: 24 Pages: 4776-83

Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB.
eLife
Eicher T, Seeger MA, Anselmi C, Zhou W, Brandstätter L, Verrey F, Diederichs K, Faraldo-Gómez JD, Pos KM PubMed
Sep 2014 Volume: 3 Issue: Pages:

Switch-loop flexibility affects transport of large drugs by the promiscuous AcrB multidrug efflux transporter.
Antimicrobial agents and chemotherapy
Cha HJ, Müller RT, Pos KM PubMed
Aug 2014 Volume: 58 Issue: 8 Pages: 4767-72

RND efflux pumps: structural information translated into function and inhibition mechanisms.
Current topics in medicinal chemistry
Ruggerone P, Murakami S, Pos KM, Vargiu AV PubMed
2013 Volume: 13 Issue: 24 Pages: 3079-100

Detecting substrates bound to the secondary multidrug efflux pump EmrE by DNP-enhanced solid-state NMR.
Journal of the American Chemical Society
Ong YS, Lakatos A, Becker-Baldus J, Pos KM, Glaubitz C PubMed
Oct 2013 Volume: 135 Issue: 42 Pages: 15754-62

The outer membrane TolC-like channel HgdD is part of tripartite resistance-nodulation-cell division (RND) efflux systems conferring multiple-drug resistance in the Cyanobacterium Anabaena sp. PCC7120.
The Journal of biological chemistry
Hahn A, Stevanovic M, Mirus O, Lytvynenko I, Pos KM, Schleiff E PubMed
Oct 2013 Volume: 288 Issue: 43 Pages: 31192-205

Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop.
Proceedings of the National Academy of Sciences of the United States of America
Eicher T, Cha HJ, Seeger MA, Brandstätter L, El-Delik J, Bohnert JA, Kern WV, Verrey F, Grütter MG, Diederichs K, Pos KM PubMed
Apr 2012 Volume: 109 Issue: 15 Pages: 5687-92

A natural prodrug activation mechanism in nonribosomal peptide synthesis.
Nature chemical biology
Reimer D, Pos KM, Thines M, Grün P, Bode HB PubMed
Sep 2011 Volume: 7 Issue: 12 Pages: 888-90

Effect of the F610A mutation on substrate extrusion in the AcrB transporter: explanation and rationale by molecular dynamics simulations.
Journal of the American Chemical Society
Vargiu AV, Collu F, Schulz R, Pos KM, Zacharias M, Kleinekathöfer U, Ruggerone P PubMed
Jul 2011 Volume: 133 Issue: 28 Pages: 10704-7

Analysis of AcrB and AcrB/DARPin ligand complexes by LILBID MS.
Biochimica et biophysica acta
Brandstätter L, Sokolova L, Eicher T, Seeger MA, Briand C, Cha HJ, Cernescu M, Bohnert J, Kern WV, Brutschy B, Pos KM PubMed
Sep 2011 Volume: 1808 Issue: 9 Pages: 2189-96

Drug resistance: a periplasmic ménage à trois.
Chemistry & biology
Oswald C, Pos KM PubMed
Apr 2011 Volume: 18 Issue: 4 Pages: 405-7

Ubiquitin-specific protease 2-45 (Usp2-45) binds to epithelial Na+ channel (ENaC)-ubiquitylating enzyme Nedd4-2.
American journal of physiology. Renal physiology
Oberfeld B, Ruffieux-Daidié D, Vitagliano JJ, Pos KM, Verrey F, Staub O PubMed
Jul 2011 Volume: 301 Issue: 1 Pages: F189-96

Structural and functional aspects of the multidrug efflux pump AcrB.
Biological chemistry
Eicher T, Brandstätter L, Pos KM PubMed
Aug 2009 Volume: 390 Issue: 8 Pages: 693-9

Crucial role of Asp408 in the proton translocation pathway of multidrug transporter AcrB: evidence from site-directed mutagenesis and carbodiimide labeling.
Biochemistry
Seeger MA, von Ballmoos C, Verrey F, Pos KM PubMed
Jun 2009 Volume: 48 Issue: 25 Pages: 5801-12

Trinity revealed: Stoichiometric complex assembly of a bacterial multidrug efflux pump.
Proceedings of the National Academy of Sciences of the United States of America
Pos KM PubMed
Apr 2009 Volume: 106 Issue: 17 Pages: 6893-4

Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations.
Gastroenterology
Camargo SM, Singer D, Makrides V, Huggel K, Pos KM, Wagner CA, Kuba K, Danilczyk U, Skovby F, Kleta R, Penninger JM, Verrey F PubMed
Mar 2009 Volume: 136 Issue: 3 Pages: 872-82

Drug transport mechanism of the AcrB efflux pump.
Biochimica et biophysica acta
Pos KM PubMed
May 2009 Volume: 1794 Issue: 5 Pages: 782-93

Site-directed mutagenesis reveals putative substrate binding residues in the Escherichia coli RND efflux pump AcrB.
Journal of bacteriology
Bohnert JA, Schuster S, Seeger MA, Fähnrich E, Pos KM, Kern WV PubMed
Dec 2008 Volume: 190 Issue: 24 Pages: 8225-9

The AcrB efflux pump: conformational cycling and peristalsis lead to multidrug resistance.
Current drug targets
Seeger MA, Diederichs K, Eicher T, Brandstätter L, Schiefner A, Verrey F, Pos KM PubMed
Sep 2008 Volume: 9 Issue: 9 Pages: 729-49

Molecular analysis of BcrR, a membrane-bound bacitracin sensor and DNA-binding protein from Enterococcus faecalis.
The Journal of biological chemistry
Gauntlett JC, Gebhard S, Keis S, Manson JM, Pos KM, Cook GM PubMed
Mar 2008 Volume: 283 Issue: 13 Pages: 8591-600

Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB.
Nature structural & molecular biology
Seeger MA, von Ballmoos C, Eicher T, Brandstätter L, Verrey F, Diederichs K, Pos KM PubMed
Feb 2008 Volume: 15 Issue: 2 Pages: 199-205

Recycling of aromatic amino acids via TAT1 allows efflux of neutral amino acids via LAT2-4F2hc exchanger.
Pflugers Archiv : European journal of physiology
Ramadan T, Camargo SM, Herzog B, Bordin M, Pos KM, Verrey F PubMed
Jun 2007 Volume: 454 Issue: 3 Pages: 507-16

Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus.
FEBS letters
Furrer EM, Ronchetti MF, Verrey F, Pos KM PubMed
Feb 2007 Volume: 581 Issue: 3 Pages: 572-8

Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism.
Science (New York, N.Y.)
Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM PubMed
Sep 2006 Volume: 313 Issue: 5791 Pages: 1295-8

Microbial drug efflux proteins of the major facilitator superfamily.
Current drug targets
Saidijam M, Benedetti G, Ren Q, Xu Z, Hoyle CJ, Palmer SL, Ward A, Bettaney KE, Szakonyi G, Meuller J, Morrison S, Pos MK, Butaye P, Walravens K, Langton K, Herbert RB, Skurray RA, Paulsen IT, O'reilly J, Rutherford NG, Brown MH, Bill RM, Henderson PJ PubMed
Jul 2006 Volume: 7 Issue: 7 Pages: 793-811

Basolateral aromatic amino acid transporter TAT1 (Slc16a10) functions as an efflux pathway.
Journal of cellular physiology
Ramadan T, Camargo SM, Summa V, Hunziker P, Chesnov S, Pos KM, Verrey F PubMed
Mar 2006 Volume: 206 Issue: 3 Pages: 771-9

Identification of a domain in the alpha-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the gamma-subunit.
The FEBS journal
Dahinden P, Pos KM, Dimroth P PubMed
Feb 2005 Volume: 272 Issue: 3 Pages: 846-55

Oxaloacetate decarboxylase of Archaeoglobus fulgidus: cloning of genes and expression in Escherichia coli.
Archives of microbiology
Dahinden P, Pos KM, Taralczak M, Dimroth P PubMed
Nov 2004 Volume: 182 Issue: 5 Pages: 414-20

An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
FEBS letters
Röthlisberger D, Pos KM, Plückthun A PubMed
Apr 2004 Volume: 564 Issue: 3 Pages: 340-8

Crystallographic analysis of AcrB.
FEBS letters
Pos KM, Schiefner A, Seeger MA, Diederichs K PubMed
Apr 2004 Volume: 564 Issue: 3 Pages: 333-9

Collection and characterisation of bacterial membrane proteins.
FEBS letters
Saidijam M, Psakis G, Clough JL, Meuller J, Suzuki S, Hoyle CJ, Palmer SL, Morrison SM, Pos MK, Essenberg RC, Maiden MC, Abu-bakr A, Baumberg SG, Neyfakh AA, Griffith JK, Stark MJ, Ward A, O'Reilly J, Rutherford NG, Phillips-Jones MK, Henderson PJ PubMed
Nov 2003 Volume: 555 Issue: 1 Pages: 170-5

Site-directed sulfhydryl labeling of the oxaloacetate decarboxylase Na+ pump of Klebsiella pneumoniae: helix VIII comprises a portion of the sodium ion channel.
Biochemistry
Wild MR, Pos KM, Dimroth P PubMed
Oct 2003 Volume: 42 Issue: 40 Pages: 11615-24

Purification, crystallization and preliminary diffraction studies of AcrB, an inner-membrane multi-drug efflux protein.
Acta crystallographica. Section D, Biological crystallography
Pos KM, Diederichs K PubMed
Oct 2002 Volume: 58 Issue: Pt 10 Pt 2 Pages: 1865-7

Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase beta subunit in the energy coupling mechanism of the Na+ pump.
European journal of biochemistry
Schmid M, Vorburger T, Pos KM, Dimroth P PubMed
Jun 2002 Volume: 269 Issue: 12 Pages: 2997-3004

Characterization of the citrate/acetate antiporter CitW of Klebsiella pneumoniae.
Archives of microbiology
Kästner CN, Schneider K, Dimroth P, Pos KM PubMed
Jun 2002 Volume: 177 Issue: 6 Pages: 500-6

Prokaryote multidrug efflux proteins of the major facilitator superfamily: amplified expression, purification and characterisation.
Journal of molecular microbiology and biotechnology
Ward A, Hoyle C, Palmer S, O'Reilly J, Griffith J, Pos M, Morrison S, Poolman B, Gwynne M, Henderson P PubMed
Apr 2001 Volume: 3 Issue: 2 Pages: 193-200

The Na+-dependent citrate carrier of Klebsiella pneumoniae: high-level expression and site-directed mutagenesis of asparagine-185 and glutamate-194.
Archives of microbiology
Kästner CN, Dimroth P, Pos KM PubMed
Volume: 174 Issue: 1-2 Pages: 67-73

The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts.
Journal of bacteriology
Pos KM, Dimroth P, Bott M PubMed
Aug 1998 Volume: 180 Issue: 16 Pages: 4160-5

Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes.
Journal of bacteriology
Schaffitzel C, Berg M, Dimroth P, Pos KM PubMed
May 1998 Volume: 180 Issue: 10 Pages: 2689-93

Functional properties of the purified Na(+)-dependent citrate carrier of Klebsiella pneumoniae: evidence for asymmetric orientation of the carrier protein in proteoliposomes.
Biochemistry
Pos KM, Dimroth P PubMed
Jan 1996 Volume: 35 Issue: 3 Pages: 1018-26

Purification of two active fusion proteins of the Na(+)-dependent citrate carrier of Klebsiella pneumoniae.
FEBS letters
Pos KM, Bott M, Dimroth P PubMed
Jun 1994 Volume: 347 Issue: 1 Pages: 37-41

Localization of peptidases in lactococci.
Applied and environmental microbiology
Tan PS, Chapot-Chartier MP, Pos KM, Rousseau M, Boquien CY, Gripon JC, Konings WN PubMed
Jan 1992 Volume: 58 Issue: 1 Pages: 285-90

Purification and characterization of an endopeptidase from Lactococcus lactis subsp. cremoris Wg2.
Applied and environmental microbiology
Tan PS, Pos KM, Konings WN PubMed
Dec 1991 Volume: 57 Issue: 12 Pages: 3593-9